Detection of Mycobacterium avium ssp. paratuberculosis in cheese, milk powder and milk using IS900 and f57-based qPCR assays
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چکیده
منابع مشابه
Detection of Mycobacterium avium subsp. paratuberculosis in Cow Milk Using Culture and PCR methods
Mycobacterium avium subspecies paratuberculosis (MAP) is the cause of John’s disease also called paratuberculosis. This is economically one of the important infectious diseases in cattle and ruminant husbandry. This disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. The typical sign of this disease is progressive loss of weight. Cons...
متن کاملMycobacterium avium subsp. paratuberculosis in powdered infant milk: F57 competitive real time PCR
Mycobacterium avium subsp. paratuberculosis (MAP) in concentrations from 48 to 32 500 cells per gram of powdered infant milk were found in 18 out of 51 investigated samples (35%) in this study. More than 10 000 cells per gram were present in four samples (7.8%). Such concentrations mean that one package of milk contains 5 million MAP cells, which are ingested by a bottle-fed baby over the cours...
متن کاملdetection of mycobacterium avium subsp. paratuberculosis in cow milk using culture and pcr methods
mycobacterium avium subspecies paratuberculosis (map) is the cause of john’s disease also called paratuberculosis. this is economically one of the important infectious diseases in cattle and ruminant husbandry. this disease is manifested as granulomatosis entrocolitis, lymphadenitis and inflammation local lymphatic vessels. the typical sign of this disease is progressive loss of weight. cons...
متن کاملDetection methods for Mycobacterium avium subsp. paratuberculosis in milk and milk products: a review
Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis, a disease with considerable economic impact, principally on dairy cattle herds. Animals with paratuberculosis shed viable MAP especially in their milk, faeces and semen. MAP may have a role in the development of Crohn’s disease in humans via the consumption of contaminated milk and milk products. The c...
متن کاملFate of Mycobacterium avium subsp. paratuberculosis in Swiss hard and semihard cheese manufactured from raw milk.
Raw milk was artificially contaminated with declumped cells of Mycobacterium avium subsp. paratuberculosis at a concentration of 10(4) to 10(5) CFU/ml and was used to manufacture model hard (Swiss Emmentaler) and semihard (Swiss Tisliter) cheese. Two different strains of M. avium subsp. paratuberculosis were tested, and for each strain, two model hard and semihard cheeses were produced. The sur...
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ژورنال
عنوان ژورنال: Journal of Applied Microbiology
سال: 2010
ISSN: 1364-5072
DOI: 10.1111/j.1365-2672.2010.04905.x